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Even when transcription by itself could have been implicated in this techniques, there is today numerous proof that the cohesin state-of-the-art plays the new significant part inside cycle extrusion. To help you analyse the newest joining away from cohesin at this engineered locus, i performed Chip-seq of your own cohesin part RAD21. The brand new endogenous RAD21 healthy protein try tagged which have a twin Strep-tag (RAD21TST) and you may Processor chip-seq try did inside the R2-installation and you will WT erythroid tissues Fig. A peak out of cohesin is seen at the R2 installation web site inside the R2-installation erythroid cells (arrow one in Fig. 3A), and this corresponds to the new unlock chromatin site understood because of ATAC-seq.